Analysis of Peptides and Proteins by Electrophoretic Techniques
Herrero-Martínez, José Manuel
Analysis of Peptides and Proteins by Electrophoretic Techniques - MDPI - Multidisciplinary Digital Publishing Institute 2019 - 1 electronic resource (110 p.)
Open Access
The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.
Creative Commons
English
books978-3-03921-228-6 9783039212279 9783039212286
10.3390/books978-3-03921-228-6 doi
seed phosphoproteomics LC-ESI-MS purity transferrin CE-LIF enzyme assay thioglucosidase capillary electrophoresis venom composition immunoassay fluorescence seed proteomics SEC-HPLC desulfo-sinigrin metalloproteins seed quality traits seed glycoproteomics proteomics sulfatase on-gel detection non-covalent binding Naja ashei myrosinase SDS-PAGE carbon dots 2-D electrophoresis fragment rhIL-12 seed molecular breeding chip-based CE-LIF assay
Analysis of Peptides and Proteins by Electrophoretic Techniques - MDPI - Multidisciplinary Digital Publishing Institute 2019 - 1 electronic resource (110 p.)
Open Access
The characterization of peptides and proteins is central to understanding their function and expression in biological matrices. Moreover, these macromolecules are important biomarkers of many human diseases. In recent years, the performance of separation techniques based on electromigration have significantly increased. The development of microdevices has reduced sample consumption and waste production while high-sensitivity detectors, such as mass spectrometry (MS) or laser-induced fluorescence (LIF), have significantly improved with regards to separation efficiency and detection limits. All of these advancements have led to appreciably enlarged fields of application. Nowadays, a multitude of studies using separation techniques based on electromigration to study proteins and peptides from numerous real matrices are available in the literature. This Special Issue covers the most recent knowledge and advances in the study of peptides and proteins using several electrophoresis techniques, as well as the characterization of relevant proteins and peptides in application areas such as clinical studies, functional foods, and toxicology.
Creative Commons
English
books978-3-03921-228-6 9783039212279 9783039212286
10.3390/books978-3-03921-228-6 doi
seed phosphoproteomics LC-ESI-MS purity transferrin CE-LIF enzyme assay thioglucosidase capillary electrophoresis venom composition immunoassay fluorescence seed proteomics SEC-HPLC desulfo-sinigrin metalloproteins seed quality traits seed glycoproteomics proteomics sulfatase on-gel detection non-covalent binding Naja ashei myrosinase SDS-PAGE carbon dots 2-D electrophoresis fragment rhIL-12 seed molecular breeding chip-based CE-LIF assay