TY  - GEN
AU  - Hong,Seok Hoon
TI  - Cell-Free Synthetic Biology
SN  - books978-3-03928-023-0
PY  - 2020///
PB  - MDPI - Multidisciplinary Digital Publishing Institute
KW  - genomically engineered E. coli
KW  - n/a
KW  - in vitro transcription-translation (TX-TL)
KW  - tPa
KW  - tissue plasminogen activator
KW  - in vitro translation
KW  - enzymes
KW  - DNA origami
KW  - antimicrobials
KW  - cell-free metabolic engineering (CFME)
KW  - cell-free transcription-translation
KW  - in vitro protein synthesis
KW  - cell-free metabolic engineering
KW  - E. coli crude extract preparation
KW  - chaperones
KW  - ischemic stroke
KW  - microsomes
KW  - sonication
KW  - cell-free protein synthesis
KW  - growth factors
KW  - artificial cell
KW  - unnatural protein
KW  - cell-free synthetic biology
KW  - rapid prototyping
KW  - drug development
KW  - unnatural amino acid
KW  - eukaryotic lysates
KW  - cell-free protein expression (CFPE)
KW  - riboregulator
KW  - synthetic biology
KW  - mathematical model
KW  - CFPS
KW  - cell-free protein synthesis (CFPS)
KW  - bioconversion platform
KW  - colicins
KW  - protein production
N1  - Open Access
N2  - Cell-free synthetic biology is in the spotlight as a powerful and rapid approach to characterize and engineer natural biological systems. The open nature of cell-free platforms brings an unprecedented level of control and freedom for design compared to in vivo systems. This versatile engineering toolkit is used for debugging biological networks, constructing artificial cells, screening protein library, prototyping genetic circuits, developing new drugs, producing metabolites, and synthesizing complex proteins including therapeutic proteins, toxic proteins, and novel proteins containing non-standard (unnatural) amino acids. The book consists of a series of reviews, protocols, benchmarks, and research articles describing the current development and applications of cell-free synthetic biology in diverse areas
UR  - https://www.mdpi.com/books/pdfview/book/1929
UR  - https://directory.doabooks.org/handle/20.500.12854/42862
ER  -