TY  - GEN
AU  - Gonçalves,Moreno
AU  - Wysllas,de Freitas Oliveira
AU  - Castelo,Branco
AU  - Laura,Araújo
AU  - Maria,Queiroz
AU  - Tavares,Donato
AU  - José,da Silva Júnior
AU  - Thays,da Silva Rodrigues
AU  - Sousa,Silva
TI  - Chapter Cell Culture and Maintenance of the Evolutionary Forms of Trypanosoma cruzi for Studies of Parasitic Biology
SN  - intechopen.84733
PY  - 2019///
PB  - InTechOpen
KW  - Medical parasitology
KW  - bicssc
KW  - Trypanosoma cruzi, Chagas disease, cell culture, in vitro assay, metacyclogenesis
N1  - Open Access
N2  - This chapter aims to present and discuss the main cell culture techniques used for the growth and maintenance of the different evolutionary forms of the protozoan Trypanosoma cruzi, the etiologic agent of the Chagas disease. Chagas disease is a neglected tropical disease endemic in several Latin American countries. Here, we intend to present the main difficulties, advantages, and disadvantages of using Trypanosoma cruzi cell culture in parasitic biology. Finally, we present the main research opportunities in the context of Trypanosoma cruzi parasitic biology using cell culture techniques, such as drug development, characterization of pharmacological targets, molecular markers for diagnosis, structural biology, and many other biomedical applications
UR  - https://library.oapen.org/bitstream/20.500.12657/49333/1/65997.pdf
UR  - https://library.oapen.org/bitstream/20.500.12657/49333/1/65997.pdf
UR  - https://library.oapen.org/bitstream/20.500.12657/49333/1/65997.pdf
UR  - https://library.oapen.org/bitstream/20.500.12657/49333/1/65997.pdf
UR  - https://directory.doabooks.org/handle/20.500.12854/70340
ER  -