Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies
Aditi Singh
Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies - IntechOpen 2019 - 1 electronic resource (134 p.)
Open Access
RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.
All rights reserved
English
intechopen.75144 9781838806972 9781838806989 9781838806965
10.5772/intechopen.75144 doi
Life Sciences Molecular Genetics Genetics and Molecular Biology Genomics Biochemistry
Modulating Gene Expression - Abridging the RNAi and CRISPR-Cas9 Technologies - IntechOpen 2019 - 1 electronic resource (134 p.)
Open Access
RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.
All rights reserved
English
intechopen.75144 9781838806972 9781838806989 9781838806965
10.5772/intechopen.75144 doi
Life Sciences Molecular Genetics Genetics and Molecular Biology Genomics Biochemistry