Entwicklung eines mikrofluidischen Zweikammer-Chipsystems mit integrierter Sensorik für die Anwendung in der Tumorforschung

By: Material type: ArticleArticleLanguage: German Publication details: KIT Scientific Publishing 2014Description: 1 electronic resource (IX, 163 p. p.)ISBN:
  • KSP/1000041112
  • 9783731502203
Subject(s): Online resources: Summary: We have developed a microfluidic two-layered channel system based on polycarbonate to investigate the transmigration of cancer cells under flow conditions and defined shear rate using live cell microscopy. The two layers are separated by a porous membrane with an endothelial monolayer on top of the membrane to mimic the blood vessel wall. To extend the measuring capabilities of our microfluidic chip, gold electrodes were structured on the membrane using optical lithography and chemical etching.
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We have developed a microfluidic two-layered channel system based on polycarbonate to investigate the transmigration of cancer cells under flow conditions and defined shear rate using live cell microscopy. The two layers are separated by a porous membrane with an endothelial monolayer on top of the membrane to mimic the blood vessel wall. To extend the measuring capabilities of our microfluidic chip, gold electrodes were structured on the membrane using optical lithography and chemical etching.

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German

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